Provitro’s endothelial cell growth media were developed to provide endothelial cells with optimal growth conditions in a low-serum environment. Provitro’s basal growth media will be supplemented with FCS less than 5%, an endothelial growth supplement, rec. human EGF and bFGF and hydrocortisone according to the specification of the growth media. Based on the expertise of provitro and some of our customers, our two endothelial cell growth media are recommended for the cell types listed below:
Endothelial cell growth medium, low serum
Endothelial cell growth medium, advanced, low serum
Microvascular endothelial cell growth medium, low serum
Microvascular endothelial cell growth medium, advanced, low serum
HUVEC, human pulmonary artery and human saphenous vein endothelial cells and endothelial cells derived from mouse, rat, rabbit, pig or cattle.
Microvascular endothelial cell growth medium, low serum
Microvascular endothelial cell growth medium, advanced, low serum
HMVEC; human coronary, lung, brain and aortic endothelial cells. Also useful with endothelial cells derived from mouse, rat, rabbit, pig or cattle.
Endothelial cell growth medium, basal OFFER NO. PRODUCT SPECIFICATION DATA SHEET 200 0001 Endothelial cell proliferation medium, basal Basal 200 0001-prf Endothelial cell proliferation medium, basal, phenol red free Basal-prf
Endothelial cell proliferation medium OFFER NO. PRODUCT SPECIFICATION DATA SHEET 201 0001 Endothelial cell proliferation medium FCS 201 0001-prf Endothelial cell proliferation medium, phenol red free FCS-prf 211 0001 Endothelial cell proliferation medium FCS kit 211 0001-prf Endothelial cell proliferation medium, phenol red free FCS kit-prf
Supplementation with SUBSTANCE FINAL CONC./ML GROWTH MEDIUM FCS 0.07 ml Heparin 10.00 µg Human rec. EGF 10.00 ng Human rec. bFGF 5.00 ng Human rec. R3 IGF-1 5.00 ng Human rec. VEGF 0.50 ng Ascorbic acid 1.00 µg Hydrocortisone 0.20 µg Gentamicin 50.00 µg Amphotericin B 50.00 µg
Endothelial cell growth medium, advanced OFFER NO. PRODUCT SPECIFICATION DATA SHEET 201 1101 Endothelial cell growth medium, advanced FCS 201 1101-prf Endothelial cell growth medium, advanced, phenol red free FCS prf 211 1101 Endothelial cell growth medium, advanced FCS kit 211 1101-prf Endothelial cell growth medium, advanced, phenol red free FCS kit-prf 212 1101 Endothelial cell growth medium, advanced HuS kit 212 1101-prf Endothelial cell growth medium, advanced, phenol red free HuS kit-prf 262 1101 Endothelial cell growth medium, GMP HuS kit On Request
Supplementation with SUBSTANCE FINAL CONC./ML GROWTH MEDIUM FCS 0.02 ml Heparin 22.50 µg Human rec. EGF 5.00 ng Human rec. bFGF 10.00 ng Human rec. VEGF 0.50 ng Human rec. R3 IGF-1 20.00 ng Ascorbic acid 1.00 µg Hydrocortisone 0.20 µg Gentamicin 50.00 µg Amphotericin B 50.00 µg
Microvascular endothelial cell growth medium, basal OFFER NO. PRODUCT SPECIFICATION DATA SHEET 200 0102 Microvascular endothelial cell growth medium, basal Basal 200 0102-prf Microvascular endothelial cell growth medium, basal, phenol red free Basal-prf
Microvascular endothelial cell growth medium OFFER NO. PRODUCT SPECIFICATION DATA SHEET 201 0102 Microvascular endothelial cell growth medium FCS 201 0102-prf Microvascular endothelial cell growth medium, phenol red free FCS-prf 211 0102 Microvascular endothelial cell growth medium FCS kit 211 0102-prf Microvascular endothelial cell growth medium, phenol red free FCS kit-prf 212 0102 Microvascular endothelial cell growth medium HuS kit 212 0102-prf Microvascular endothelial cell growth medium, phenol red free HuS kit-prf
Supplementation with SUBSTANCE FINAL CONC./ML GROWTH MEDIUM FCS 0.05 ml ECGS/H (3 mg/ml) 4.00 µl human rec. EGF 10.00 ng Hydrocortison 1.00 µg Gentamicin 50.00 µg Amphotericin B 50.00 µg
Microvascular endothelial cell growth medium, advanced OFFER NO. PRODUCT SPECIFICATION DATA SHEET 201 1102 Microvascular endothelial cell growth medium, advanced FCS 201 1102-prf Microvascular endothelial cell growth medium, advanced, phenol red free FCS-prf 211 1102 Microvascular endothelial cell growth medium, advanced FCS kit 211 1102-prf Microvascular endothelial cell growth medium, advanced, phenol red free FCS kit-prf 212 1102 Microvascular endothelial cell growth medium, advanced HuS kit 212 1102-prf Microvascular endothelial cell growth medium, advanced, phenol red free HuS kit-prf 262 1102 Microvascular endothelial cell growth medium, GMP HuS kit On request
Supplementation with SUBSTANCE FINAL CONC./ML GROWTH MEDIUM FCS 0.05 ml human rec. EGF 5.00 ng human rec. bFGF 10.00 ng human rec. R3 IGF-1 20.00 ng human rec. VEGF 0.50 ng Ascorbic acid 1.00 µg Hydrocortison, 0.1 mg 0.20 µg Gentamicin 50.00 µg Amphotericin B 50.00 µg