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灌流培養系

perfusion circuit

Perfusion circuit PCS3c

In the case of different culture media flowing through the upper and lower chambers, a multi-channel pump is used to generate similar flow rates in both circuits. Alternatively, the flow from a single outlet from the media reservoir may be split by using Y-connectors. For convenience, both circuits may be merged after leaving the chamber using a Y-connector.

Perfusion circuit TCS2c

Different flow rates in the inner and outer circuits can be generated using different diameters of tubing. Using sterile couplings mounted between the Luer connectors, the media bag can be changed without interrupting the media flow.

Perfusion circuit FCS1c

In this open circuit, the culture medium passes through the flow chamber at a shear rate defined by the pump and is collected in another reservoir. The culture medium is pre-conditioned in a gas-permeable media bag by equilibrating in a CO2 incubator. Using impermeable tubing, the partial gas pressure remains stable until the medium reaches the cell monolayer.

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Circuit part list
OFFER NO.PRODUCTSPECIFICATION
300 0101Silicone tubing, Luer fitting on each sidei.d = 1 mm; l = 1 m
300 0102Silicone tubing, Luer fitting on each sidei.d = 3 mm; l = 1 m
300 0210Luer tubing adapter, male and female, 5 eachi.d. = 1.6 mm
300 0220Luer tubing adapter, male and female, 5 eachi.d. = 3.2 mm
300 0303Gas-permeable media bagV = 1000 ml
300 0400Sterile Luer coupling, pack of 10
300 0511Pump ISMATEC IPC-40.002 ... 44 ml/min
300 0512Pump ISMATEC IPC-N40.0004 ... 11 ml/min
300 0513Pump ISMATEC IPC-80.002 ... 44 ml/min
300 0514Pump ISMATEC IPC-N80.0004 ... 11 ml/min
300 0521Pump tubing, with 2 stoppers, Luer fitting on each sidei.d. = 1 mm
300 0522Pump tubing, with 2 stoppers, Luer fitting on each sidei.d. = 3 mm
300 0710Y-connector, pack of 2i.d. = 1.6 mm
300 0720Y-connector, pack of 2i.d. = 3.2 mm
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